Cell Lines and Cell Cultures
Eurofins Discoverx Ready-to-Assay CCR4 Chemokine Receptor Frozen Cells, 2 Vials of Cells. (HTS009RTA)
The Ready-to-Assay CCR4 Chemokine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. MIP, RANTES) based activation of CCR4 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CCR4 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CCR4, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen CCR10 Chemokine Receptor Stable Cell Line, 2 vials. (HTS014C)
The ChemiScreen CCR10 Chemokine Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CCL27) based activation of CCR10 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CCR10 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CCR10, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx Ready-to-Assay CB1 Cannabinoid Receptor Frozen Cells, 2 Vials of Cells. (HTS019RTA)
The Ready-to-Assay CB1 Cannabinoid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. cannabinoids, CP 55,940) based activation of CNR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CNR1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CNR1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay CCR10 Chemokine Receptor Frozen Cells, 2 Vials of Cells. (HTS014RTA)
The Ready-to-Assay CCR10 Chemokine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. fractalkine) based activation of CCR10 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CCR10 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CCR10, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen δ Opioid Receptor Stable Cell Line, 2 vials. (HTS100C)
The ChemiScreen δ Opioid Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. DADLE, DPDPE) based activation of OPRD1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses OPRD1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of OPRD1, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen CXCR5 Chemokine Receptor Stable Cell Line, 2 vials. (HTS055C)
The ChemiScreen CXCR5 Chemokine Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CXCL13/BCA-1/BLC) based activation of CXCR5 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CXCR5 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CXCR5, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx Ready-to-Assay SST4 Somatostatin Receptor Frozen Cells, 2 Vials of Cells. (HTS125RTA)
The Ready-to-Assay SST4 Somatostatin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. somatostatin) based activation of SSTR4 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses SSTR4 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of SSTR4, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay GnRH Gonadotropin-Releasing Receptor Frozen Cells, 2 Vials of Cells. (HTS027RTA)
The Ready-to-Assay GnRH Gonadotropin-Releasing Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. gonadotropin-releasing hormone) based activation of GNRHR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses GNRHR alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of GNRHR, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay CXCR5 Chemokine Receptor Frozen Cells, 2 Vials of Cells. (HTS055RTA)
The Ready-to-Assay CXCR5 Chemokine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. CXCL13/BCA-1/BLC) based activation of CXCR5 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CXCR5 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CXCR5, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay CCR9 Chemokine Receptor Frozen Cells, 2 Vials of Cells. (HTS036RTA)
The Ready-to-Assay CCR9 Chemokine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. CCL25/TECK) based activation of CCR9 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CCR9 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CCR9, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay ETB Endothelin Receptor Frozen Cells, 2 Vials of Cells. (HTS046RTA)
The Ready-to-Assay ETB Endothelin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. endothelin) based activation of EDNRB GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses EDNRB alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of EDNRB, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen AT1 Angiotensin Receptor Membrane Preparation, 1 vial. (HTS064M)
The ChemiScreen AT1 Angiotensin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of AGTR1 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen NMU1 Neuromedin Receptor Membrane Preparation, 1 vial. (HTS062M)
The ChemiScreen nMU1 Neuromedin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of nMU1R surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx PrecisION hnAChR α1/β1/δ/ε Recombinant Stable Cell Line, 2 vials. (CYL3052)
The PrecisION hnAChR α1/β1/δ/ε Recombinant Stable Cell Line is a stable, engineered cell line for use in studying nAChR α1/β1/δ/ε ion channels, including drug candidates which affect channel function. This cell line enables assessment of ion channel function via detection of electrophysiological signals. The included cell line overexpresses nAChR α1/β1/δ/ε ion channels. Upon ligand/agonist induced channel activation, response can be detected via automated or manual electrophysiological techniques. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/ion-channels.